验证数据展示
经过测试的应用
| Positive WB detected in | NIH/3T3 cells, HepG2 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14491-1-AP targets RPS7 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RPS7 fusion protein Ag5892 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S7 |
| 别名 | 40S ribosomal protein S7, DBA8, ribosomal protein S7, RPS 7, Small ribosomal subunit protein eS7 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22-25 kDa |
| GenBank蛋白编号 | BC061901 |
| 基因名称 | RPS7 |
| Gene ID (NCBI) | 6201 |
| RRID | AB_11232411 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62081 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ribosomal protein S7(RPS7) belongs to the RPs, which is structural compoent of the ribosome invilved in protein synthesis. It interacts with and inhibit mdm2 mediated p53 degradation function. Also RPS7 has a essential role in early development. RPS7 involves in the maturation of ribosomal RNAs in the large or the small ribosomal subunit production pathway
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS7 antibody 14491-1-AP | Download protocol |
| IHC protocol for RPS7 antibody 14491-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Identifying the functional contribution of the defatty-acylase activity of SIRT6. | ||
Sci Rep Stabilization of SIRT7 deacetylase by viral oncoprotein HBx leads to inhibition of growth restrictive RPS7 gene and facilitates cellular transformation. | ||
Exp Cell Res Combination of DESI2 and endostatin gene therapy significantly improves antitumor efficacy by accumulating DNA lesions, inducing apoptosis and inhibiting angiogenesis. | ||
Cell Death Dis SMYD2 promotes tumorigenesis and metastasis of lung adenocarcinoma through RPS7. | ||
Biomed Res Int Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury
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