S6K2 Polyclonal antibody
S6K2 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | MCF7 cells, MCF-7 cells |
Positive IP detected in | MCF-7 cells |
Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IHC | See 1 publications below |
产品信息
15268-1-AP targets S6K2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | S6K2 fusion protein Ag7371 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | ribosomal protein S6 kinase, 70kDa, polypeptide 2 |
Synonyms | KLS, P70 beta, P70 beta 1, P70 beta 2, p70 ribosomal S6 kinase beta, P70 S6 kinase beta, p70 S6K 2, p70 S6K beta, p70 S6KB, p70(S6K) beta, P70S6K2, p70S6Kb, RPS6KB2, S6 kinase related kinase, S6K beta, S6K beta 2, S6K beta2, S6K2, SRK, STK14B |
Calculated Molecular Weight | 53 kDa |
Observed Molecular Weight | 54 kDa |
GenBank Accession Number | BC000094 |
Gene Symbol | RPS6KB2 |
Gene ID (NCBI) | 6199 |
RRID | AB_2182250 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UBS0 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for S6K2 antibody 15268-1-AP | Download protocol |
IHC protocol for S6K2 antibody 15268-1-AP | Download protocol |
IF protocol for S6K2 antibody 15268-1-AP | Download protocol |
IP protocol for S6K2 antibody 15268-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis. | ||
J Ethnopharmacol Exploring the potential mechanism of Heng-Gu-Gu-Shang-Yu-He-Ji therapy for osteoporosis based on network pharmacology and transcriptomics |