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验证数据展示

  • IHC staining of human lung cancer using 15977-1-AP

    IHC staining of human lung cancer using 15977-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15977-1-AP (MSK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 15977-1-AP

    IHC staining of human lung cancer using 15977-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15977-1-AP (MSK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 15977-1-AP

    IF Staining of MCF-7 using 15977-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MSK1 antibody (15977-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

MSK1 Polyclonal antibody

MSK1 Polyclonal Antibody for IF, IHC, ELISA
Cat No. 15977-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF, IHC, ELISA

别名

MSK1, MSPK1, RLPK, RPS6KA5, RSK like protein kinase, RSKL, S6K alpha 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15977-1-AP targets MSK1 in IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 MSK1 fusion protein Ag8765 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S6 kinase, 90kDa, polypeptide 5
别名 MSK1, MSPK1, RLPK, RPS6KA5, RSK like protein kinase, RSKL, S6K alpha 5
计算分子量549aa,62 kDa; 802aa,90 kDa
GenBank蛋白编号BC017187
基因名称 MSK1
Gene ID (NCBI) 9252
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75582
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.