验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy RPS5 Ready-To-Use IHC Kit. RPS5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IF | See 1 publications below |
产品信息
16964-1-AP targets RPS5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat, c.elegans |
| 免疫原 |
CatNo: Ag10686 Product name: Recombinant human RPS5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC018151 Sequence: MTEWETAAPAVAETPDIKLFGKWSTDDVQINDISLQDYIAVKEKYAKYLPHSAGRYAAKRFRKAQCPIVERLTNSMMMHGRNNGKKLMTVRIVKHAFEIIHLLTGENPLQVLVNAIINSGPREDSTRIGRAGTVRRQAVDVSPLRRVNQAIWLLCTGAREAAFRNIKTIAECLADELINAAKGSSNSYAIKKKDELERVAKSNR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S5 |
| 别名 | Small ribosomal subunit protein uS7, N-terminally processed, Small ribosomal subunit protein uS7, ribosomal protein S5 |
| 计算分子量 | 204 aa, 23 kDa |
| 观测分子量 | 23 kDa |
| GenBank蛋白编号 | BC018151 |
| 基因名称 | RPS5 |
| Gene ID (NCBI) | 6193 |
| RRID | AB_2180894 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P46782 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS5 antibody 16964-1-AP | Download protocol |
| IHC protocol for RPS5 antibody 16964-1-AP | Download protocol |
| IF protocol for RPS5 antibody 16964-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Struct Mol Biol Rps26 directs mRNA-specific translation by recognition of Kozak sequence elements. | ||
J Cell Biol Ribosome biogenesis factor Ltv1 chaperones the assembly of the small subunit head. | ||
Elife A RanGTP-independent mechanism allows ribosomal protein nuclear import for ribosome assembly. | ||
J Biochem Mol Toxicol Matrine alleviates hypoxia-induced inflammation and pulmonary vascular remodelling via RPS5/NF-κB signalling pathway | ||
