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验证数据展示

  • WB analysis using 86254-3-RR (same clone as 86254-3-PBS)

    WB analysis using 86254-3-RR (same clone as 86254-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86254-3-RR (RPS3A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86254-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86254-3-RR (same clone as 86254-3-PBS)

    IF Staining of HepG2 using 86254-3-RR (same clone as 86254-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPS3A antibody (86254-3-RR, Clone: 251033B11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86254-3-PBS in a different storage buffer formulation.

RPS3A Recombinant monoclonal antibody, PBS Only

RPS3A Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86254-3-PBS

产品说明书

CloneNo. 251033B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

40S ribosomal protein S3a, Fte 1, FTE1, Fte-1, MFTL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy RPS3A Ready-To-Use IHC Kit. RPS3A primary antibody included.

产品信息

86254-3-PBS targets RPS3A in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5273

Product name: Recombinant human RPS3A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-264 aa of BC066926

Sequence: MAVSKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein S3A
别名 40S ribosomal protein S3a, Fte 1, FTE1, Fte-1, MFTL
计算分子量 30 kDa
观测分子量 35 kDa
GenBank蛋白编号BC066926
基因名称 RPS3A
Gene ID (NCBI) 6189
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP61247
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors.

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