验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A431 cells, Jurkat cells, A549 cells, HepG2 cells, Raji cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14123-1-AP targets RPS3A in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, xenopus |
| 免疫原 |
CatNo: Ag5273 Product name: Recombinant human RPS3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-264 aa of BC066926 Sequence: MAVSKNKRLTKGGKKGAKKKVVDPFSKKDWYDVKAPAMFNIRNIGKTLVTRTQGTKIASDGLKGRVFEVSLADLQNDEVAFRKFKLITEDVQGKNCLTNFHGMDLTRDKMCSMVKKWQTMIEAHVDVKTTDGYLLRLFCVGFTKKRNNQIRKTSYAQHQQVRQIRKKMMEIMTREVQTNDLKEVVNKLIPDSIGKDIEKACQSIYPLHDVFVRKVKMLKKPKFELGKLMELHGEGSSSGKATGDETGAKVERADGYEPPVQESV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S3A |
| 别名 | 40S ribosomal protein S3a, Fte 1, FTE1, Fte-1, MFTL |
| 计算分子量 | 30 kDa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | BC066926 |
| 基因名称 | RPS3A |
| Gene ID (NCBI) | 6189 |
| RRID | AB_2253921 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P61247 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPS3a, a component of the ribosomal small subunit (40S), is a member of the A20 family of the deubiquitinating cysteine proteases. RPS3a is an inducing factor for oncogenic cellular transformation of Rat-1 cells by v-fos, and is highly expressed in most tumors including hepatocellular carcinoma and other cancers. On the other hand, RPS3a has also been described as a regulator of apoptosis and transcription factors.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS3A antibody 14123-1-AP | Download protocol |
| IHC protocol for RPS3A antibody 14123-1-AP | Download protocol |
| IP protocol for RPS3A antibody 14123-1-AP | Download protocol |
| WB protocol for RPS3A antibody 14123-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function. | ||
Mol Cell Proteomics Proteomic Profiling Identified Multiple Short-lived Members of the Central Proteome as the Direct Targets of the Addicted oncogenes in Cancer Cells. | ||
Mol Cell Proteomics Proteomic landscape of exosomes reveals the functional contributions of CD151 in triple-negative breast cancer. | ||
Cell Discov RPS3A positively regulates the mitochondrial function of human periaortic adipose tissue and is associated with coronary artery diseases.
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