验证数据展示
经过测试的应用
| Positive WB detected in | SH-SY5Y cells, HEK-293 cells, HL-60 cells |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 2 publications below |
产品信息
14831-1-AP targets RPS24 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag6621 Product name: Recombinant human RPS24 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-130 aa of BC000523 Sequence: MNDTVTIRTRKFMTNRLLQRKQMVIDVLHPGKATVPKTEIREKLAKMYKTTPDVIFVFGFRTHFGGGKTTGFGMIYDSLDYAKKNEPKHRLARHGLYEKKKTSRKQRKERKNRMKKVRGTAKANVGAGKK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S24 |
| 别名 | Small ribosomal subunit protein eS24, RPS 24, ribosomal protein S24, DBA3, 40S ribosomal protein S24 |
| 计算分子量 | 15 kDa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | BC000523 |
| 基因名称 | RPS24 |
| Gene ID (NCBI) | 6229 |
| RRID | AB_2878084 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62847 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
40S ribosomal protein S24 (RPS24) is a 133 amino acid protein, which belongs to the ribosomal protein S24e family. RPS24 is required for processing of pre-rRNA and maturation of 40S ribosomal subunits. RPS24 has an association with Diamond-Blackfan anemia 3.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS24 antibody 14831-1-AP | Download protocol |
| WB protocol for RPS24 antibody 14831-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Ecotoxicol Environ Saf ZEA and DON inhibited inflammation after L. monocytogenes infection and induced ribosomal hyperfunction. | ||
Int J Mol Sci RPS24 Is Associated with a Poor Prognosis and Immune Infiltration in Hepatocellular Carcinoma | ||
Cancer Immunol Immunother Integrating bulk and single-cell sequencing data to construct a Scissor+ dendritic cells prognostic model for predicting prognosis and immune responses in ESCC |
