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RPS15A Polyclonal antibody

RPS15A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14942-1-AP

Print datasheet

Synonyms

40S ribosomal protein S15a, ribosomal protein S15a, RPS15A, S15a

验证数据展示

  • IHC staining of human kidney using 14942-1-AP

    IHC staining of human kidney using 14942-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14942-1-AP

    WB analysis using 14942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14942-1-AP (RPS15A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14942-1-AP

    IHC staining of human kidney using 14942-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14942-1-AP targets RPS15A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen RPS15A fusion protein Ag6655 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S15a
Synonyms 40S ribosomal protein S15a, ribosomal protein S15a, RPS15A, S15a
Calculated Molecular Weight 15 kDa
Observed Molecular Weight13-15 kDa
GenBank Accession NumberBC001697
Gene Symbol RPS15A
Gene ID (NCBI) 6210
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62244
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS15A is a component of the small ribosomal subunit, it is required for proper erythropoiesis.

实验方案

Product Specific Protocols
WB protocol for RPS15A antibody 14942-1-APDownload protocol
IHC protocol for RPS15A antibody 14942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols