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验证数据展示

  • IHC staining of human kidney using 14942-1-AP

    IHC staining of human kidney using 14942-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 14942-1-AP

    WB analysis using 14942-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14942-1-AP (RPS15A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14942-1-AP

    IHC staining of human kidney using 14942-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14942-1-AP (RPS15A antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RPS15A Polyclonal antibody

RPS15A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14942-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

40S ribosomal protein S15a, ribosomal protein S15a, RPS15A, S15a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14942-1-AP targets RPS15A in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag6655

Product name: Recombinant human RPS15A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-130 aa of BC001697

Sequence: MVRMNVLADALKSINNAEKRGKRQVLIRPCSKVIVRFLTVMMKHGYIGEFEIIDDHRAGKIVVNLTGRLNKCGVISPRSDVQLKDLEKWQNNLLPSRQFGFIVLTTSAGIMDHEEARRKHTGGKILGFFF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein S15a
别名 40S ribosomal protein S15a, ribosomal protein S15a, RPS15A, S15a
计算分子量 15 kDa
观测分子量13-15 kDa
GenBank蛋白编号BC001697
基因名称 RPS15A
Gene ID (NCBI) 6210
RRIDAB_3085446
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP62244
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS15A is a component of the small ribosomal subunit, it is required for proper erythropoiesis.

实验方案

Product Specific Protocols
WB protocol for RPS15A antibody 14942-1-APDownload protocol
IHC protocol for RPS15A antibody 14942-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cancer Med

MCM8 promotes gastric cancer progression through RPS15A and predicts poor prognosis

Authors - Lixian Ding
View Article