RPS15 Polyclonal antibody

RPS15 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14957-1-AP

Print datasheet

Synonyms

40S ribosomal protein S15, ribosomal protein S15, RIG, RIG protein, RPS15



经过测试的应用

Positive WB detected inhuman liver tissue, HeLa cells, L02 cells, mouse brain tissue, mouse liver tissue
Positive IHC detected inhuman lymphoma tissue, mouse liver tissue, rat liver tissue, human liver cancer tissue, human colon cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy RPS15 Ready-To-Use IHC Kit. RPS15 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:400-1:1600
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14957-1-AP targets RPS15 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RPS15 fusion protein Ag6795 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein S15
Synonyms 40S ribosomal protein S15, ribosomal protein S15, RIG, RIG protein, RPS15
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC064908
Gene Symbol RPS15
Gene ID (NCBI) 6209
RRIDAB_2180163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62841
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPS15, also named as RIG protein or Small ribosomal subunit protein uS19, is a 145 amino acid protein, which belongs to the universal ribosomal protein uS19 family. RPS15 is a ribosomal small subunit assembly and involved in nuclear-transcribed mRNA catabolic process, nonsense-mediated decay.

实验方案

Product Specific Protocols
WB protocol for RPS15 antibody 14957-1-APDownload protocol
IHC protocol for RPS15 antibody 14957-1-APDownload protocol
IF protocol for RPS15 antibody 14957-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Curr Biol

RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice.

Authors - Long Jiang
humanIF

Cell Prolif

An artificial intelligence network-guided signature for predicting outcome and immunotherapy response in lung adenocarcinoma patients based on 26 machine learning algorithms

Authors - Nan Zhang
humanWB

Elife

The molecular basis of coupling between poly(A)-tail length and translational efficiency.

Authors - Kehui Xiang
human

Cell Rep

FKBP10 Regulates Protein Translation to Sustain Lung Cancer Growth.

Authors - Giorgio Ramadori
humanWB

Elife

The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1.

Authors - Diego Acosta-Alvear
humanWB

iScience

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

Authors - Magdalena Wolczyk