RPS14 Polyclonal antibody
RPS14 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy RPS14 Ready-To-Use IHC Kit. RPS14 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16683-1-AP targets RPS14 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, Arabidopsis, zebrafish |
| Immunogen | RPS14 fusion protein Ag10012 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ribosomal protein S14 |
| Synonyms | 40S ribosomal protein S14, EMTB, ribosomal protein S14, RPS14 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC006784 |
| Gene Symbol | RPS14 |
| Gene ID (NCBI) | 6208 |
| RRID | AB_2180158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62263 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS14 antibody 16683-1-AP | Download protocol |
| IHC protocol for RPS14 antibody 16683-1-AP | Download protocol |
| IF protocol for RPS14 antibody 16683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules. | ||
J Exp Bot The in vivo functions of ARPF2 and ARRS1 in ribosomal RNA processing and ribosome biogenesis in Arabidopsis. | ||
Free Radic Biol Med Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage. | ||
J Exp Bot Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice. | ||
Blood Adv TLR7 ligation augments hematopoiesis in Rps14 (uS11) deficiency via paradoxical suppression of inflammatory signaling. |
