验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67609-1-Ig targets RPS10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | RPS10 fusion protein Ag7018 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein S10 |
| 别名 | 1G1E1, ribosomal protein S10, Small ribosomal subunit protein eS10 |
| 计算分子量 | 19 kDa |
| 观测分子量 | 19 kDa |
| GenBank蛋白编号 | BC001032 |
| 基因名称 | RPS10 |
| Gene ID (NCBI) | 6204 |
| RRID | AB_2882815 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P46783 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPS10 (ribosomal protein S10) encodes a ribosomal protein that is a component of the small 40S subunit of the ribosome. RPS10 is essential for cell proliferation and methylation. In nucleoli, RPS10 interacts with nucleophosmin/B23 and is predominantly concentrated in the granular component region, which is required for ribosome assembly (PMID: 20159986, 23723321). Human RPS10 protein consists of 165 amino acids, with a molecular weight of approximately 18.5 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS10 antibody 67609-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |