验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, SK-BR-3 cells, Caco-2 cells, Calu-3 cells, BxPC-3 cells, NIH/3T3 cells, HSC-T6 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88014-2-RR targets RPN2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag40161 Product name: Recombinant human RPN2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 222-452 aa of BC003560 Sequence: VGTEPSIKEDQVIQLMNAIFSKKNFESLSEAFSVASAAAVLSHNRYHVPVVVVPEGSASDTHEQAILRLQVTNVLSQPLTQATVKLEHAKSVASRATVLQKTSFTPVGDVFELNFMNVKFSSGYYDFLVEVEGDNRYIANTVELRVKISTEVGITNVDLSTVDKDQSIAPKTTRVTYPAKAKGTFIADSHQNFALFFQLVDVNTGAELTPHQTFVRLHNQKTGQEVVFVAE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ribophorin II |
| 别名 | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, RIBIIR, Ribophorin 2, Ribophorin-2 |
| 计算分子量 | 69 kDa |
| 观测分子量 | 68-69 kDa |
| GenBank蛋白编号 | BC003560 |
| 基因名称 | RPN2 |
| Gene ID (NCBI) | 6185 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P04844 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RPN2 (Ribophorin II), an integral rough ER membrane glycoprotein, is part of an N-oligosaccharyl transferase complex and is involved in translocation and the maintenance of the structural uniqueness of the rough ER. RPN2 expression has been reported to be significantly associated with solid cancers, including breast cancer, esophageal squamous cell carcinoma, colorectal cancer, and lung squamous cell carcinoma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RPN2 antibody 88014-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
