验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse kidney tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14583-1-AP targets RPL7 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag6104 Product name: Recombinant human RPL7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-248 aa of BC006095 Sequence: MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L7 |
| 别名 | ribosomal protein L7, Large ribosomal subunit protein uL30, humL7 1, 60S ribosomal protein L7 |
| 计算分子量 | 29 kDa |
| 观测分子量 | 29-30 kDa |
| GenBank蛋白编号 | BC006095 |
| 基因名称 | RPL7 |
| Gene ID (NCBI) | 6129 |
| RRID | AB_2254049 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P18124 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL7 binds to G-rich structures in 28S rRNA, and plays a regulatory role in the translation apparatus, inhibits cell-free translation of mRNAs. In addition, RPL7 is suggested to have a role as an integral part of natural freeze tolerance[PMID: 18706984]. Besides, RPL7 is responsible for its normal ribosome functions including the nuclear targeting, ribosome assembly and translation[PMID:17258209].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL7 antibody 14583-1-AP | Download protocol |
| IHC protocol for RPL7 antibody 14583-1-AP | Download protocol |
| IP protocol for RPL7 antibody 14583-1-AP | Download protocol |
| WB protocol for RPL7 antibody 14583-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Hematol Oncol METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation | ||
Nat Cell Biol METTL16 exerts an m6A-independent function to facilitate translation and tumorigenesis. | ||
Front Endocrinol (Lausanne) Glucose controls co-translation of structurally related mRNAs via the mTOR and eIF2 pathways in human pancreatic beta cells | ||
Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function |
