验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, PC-12 cells, RAW 264.7 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15185-1-AP targets RPL36A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag7210 Product name: Recombinant human RPL36A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-106 aa of BC001781 Sequence: MVNVPKTRRTFCKKCGKHQPHKVTQYKKGKDSLYAQGKRRYDRKQSGYGGQTKPIFRKKAKTTKKIVLRLECVEPNCRSKRMLAIKRCKHFELGGDKKRKGQVIQF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L36a |
| 别名 | MIG6, Large ribosomal subunit protein eL42, L44L, GIG15, Cell migration-inducing gene 6 protein |
| 计算分子量 | 12 kDa |
| 观测分子量 | 12-17 kDa |
| GenBank蛋白编号 | BC001781 |
| 基因名称 | RPL36A |
| Gene ID (NCBI) | 6173 |
| RRID | AB_3669200 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P83881 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPL36A antibody 15185-1-AP | Download protocol |
| WB protocol for RPL36A antibody 15185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
