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验证数据展示

  • WB analysis of RAW 264.7 using 66130-1-Ig (same clone as 66130-1-PBS)

    WB analysis of RAW 264.7 using 66130-1-Ig (same clone as 66130-1-PBS)

    Western blot analysis of RPL3 in various cell lines using Proteintech antibody 66130-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66130-1-Ig (same clone as 66130-1-PBS)

    WB analysis of HEK-293 using 66130-1-Ig (same clone as 66130-1-PBS)

    Western blot analysis of RPL3 in various cell lines and tissues using Proteintech antibody 66130-1-Ig at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66130-1-Ig (same clone as 66130-1-PBS)

    IHC staining of human liver cancer using 66130-1-Ig (same clone as 66130-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66130-1-Ig (RPL3 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66130-1-Ig (same clone as 66130-1-PBS)

    IF Staining of HepG2 using 66130-1-Ig (same clone as 66130-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL3 antibody (66130-1-Ig, Clone: 2G1B8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66130-1-PBS in a different storage buffer formulation.

RPL3 Monoclonal antibody, PBS Only

RPL3 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66130-1-PBS

产品说明书

CloneNo. 2G1B8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66130-1-PBS targets RPL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag20400

Product name: Recombinant human RPL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 153-403 aa of BC012786

Sequence: MKKYCQVIRVIAHTQMRLLPLRQKKAHLMEIQVNGGTVAEKLDWARERLEQQVPVNQVFGQDEMIDVIGVTKGKGYKGVTSRWHTKKLPRKTHRGLRKVACIGAWHPARVAFSVARAGQKGYHHRTEINKKIYKIGQGYLIKDGKLIKNNASTDYDLSDKSINPLGGFVHYGEVTNDFVMLKGCVVGTKKRVLTLRKSLLVQTKRRALEKIDLKFIDTTSKFGHGRFQTMEEKKAFMGPLKKDRIAKEEGA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ribosomal protein L3
别名 60S ribosomal protein L3, ribosomal protein L3, RPL3, TARBP B
计算分子量 46/27 kDa
观测分子量 46 kDa
GenBank蛋白编号BC012786
基因名称 RPL3
Gene ID (NCBI) 6122
RRIDAB_2881529
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP39023
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. RPL3, also named as TARBP-B and MGC104284, belongs to the ribosomal protein L3P family. This protein is a component of the 60S subunit. It can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

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