验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, human liver tissue, human heart tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human normal colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy RPL23A Ready-To-Use IHC Kit. RPL23A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 8 publications below |
| RIP | See 1 publications below |
产品信息
16386-1-AP targets RPL23A in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag9424 Product name: Recombinant human RPL23A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-156 aa of BC014459 Sequence: MAPKAKKEAPAPPKAEAKAKALKAKKAVLKGVHSHKKKKIRTSPTFRRPKTLRLRRQPKYPRKSAPRRNKLDHYAIIKFPLTTESAMKKIEDNNTLVFIVDVKANKHQIKQAVKKLYDIDVAKVNTLIRPDGEKKAYVRLAPDYDALDVANKIGII 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ribosomal protein L23a |
| 别名 | 60S ribosomal protein L23a, Large ribosomal subunit protein uL23, MDA20, ribosomal protein L23a |
| 计算分子量 | 156 aa, 18 kDa |
| 观测分子量 | 18-23 kDa |
| GenBank蛋白编号 | BC014459 |
| 基因名称 | RPL23A |
| Gene ID (NCBI) | 6147 |
| RRID | AB_2269755 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62750 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPL23A antibody 16386-1-AP | Download protocol |
| IP protocol for RPL23A antibody 16386-1-AP | Download protocol |
| WB protocol for RPL23A antibody 16386-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Stem Cell HectD1 controls hematopoietic stem cell regeneration by coordinating ribosome assembly and protein synthesis. | ||
Nat Commun Kaposi's sarcoma-associated herpesvirus induces specialised ribosomes to efficiently translate viral lytic mRNAs | ||
Nat Commun The Perlman syndrome DIS3L2 exoribonuclease safeguards endoplasmic reticulum-targeted mRNA translation and calcium ion homeostasis. | ||
Brain Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA. | ||
Gene Intron retention is a stress response in sensor genes and is restored by Japanese herbal medicines: A basis for future clinical applications. | ||
Cell Death Dis HERC3 regulates epithelial-mesenchymal transition by directly ubiquitination degradation EIF5A2 and inhibits metastasis of colorectal cancer. |
