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验证数据展示

  • WB analysis using 15751-1-AP

    WB analysis using 15751-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15751-1-AP (RPL18A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of Jurkat using 15751-1-AP

    IP experiment of Jurkat using 15751-1-AP

    IP result of anti-RPL18A (IP:15751-1-AP, 4ug; Detection:15751-1-AP 1:500) with Jurkat cells lysate 3200 ug.

  • IHC staining of human brain using 15751-1-AP

    IHC staining of human brain using 15751-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15751-1-AP (RPL18A antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human brain using 15751-1-AP

    IHC staining of human brain using 15751-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15751-1-AP (RPL18A antibody) at dilution of 1:100 (under 40x lens).

RPL18A Polyclonal antibody

RPL18A Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15751-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

60S ribosomal protein L18a, ribosomal protein L18a, RPL18A

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15751-1-AP targets RPL18A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8251

Product name: Recombinant human RPL18A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-176 aa of BC007512

Sequence: MKASGTLREYKVVGRCLPTPKCHTPPLYRMRIFAPNHVVAKSRFWYFVSQLKKMKKSSGEIVYCGQVFEKSPLRVKNFGIWLRYDSRSGTHNMYREYRDLTTAGAVTQCYRDMGARHRARAHSIQIMKVEEIAASKCRRPAVKQFHDSKIKFPLPHRVLRRQHKPRFTTKRPNTFF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L18a
别名 60S ribosomal protein L18a, ribosomal protein L18a, RPL18A
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC007512
基因名称 RPL18A
Gene ID (NCBI) 6142
RRIDAB_2181574
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02543
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for RPL18A antibody 15751-1-APDownload protocol
IP protocol for RPL18A antibody 15751-1-APDownload protocol
WB protocol for RPL18A antibody 15751-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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