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验证数据展示

  • WB analysis using 82688-1-RR

    WB analysis using 82688-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82688-1-RR (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 82688-1-RR

    IF Staining of HepG2 using 82688-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL14 antibody (82688-1-RR, Clone: 2K22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Affinity and Kinetic Characterization of 82688-1-RR

    Affinity and Kinetic Characterization of 82688-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82688-1-RR against Human RPL14 were performed. The affinity constant is below 1 pM.

RPL14 Recombinant monoclonal antibody

RPL14 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82688-1-RR

产品说明书

CloneNo. 2K22

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

2K22, CAG ISL 7, CAG-ISL 7, CTG B33, hRL14

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, MCF-7 cells, A431 cells, MDA-MB-453s cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL14 Ready-To-Use IHC Kit. RPL14 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82688-1-RR targets RPL14 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag6976

Product name: Recombinant human RPL14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-216 aa of BC000606

Sequence: MVFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEVKKLQKAALLKASPKKAPGTKGTAAAAAAAAAAAKVPAKKITAASKKAPAQKVPAQKATGQKAAPAPKAQKGQKAPAQKAPAPKASGKKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ribosomal protein L14
别名 2K22, CAG ISL 7, CAG-ISL 7, CTG B33, hRL14
计算分子量 23 kDa
观测分子量23-27 kDa
GenBank蛋白编号BC000606
基因名称 RPL14
Gene ID (NCBI) 9045
RRIDAB_3086505
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP50914
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.

实验方案

Product Specific Protocols
IF protocol for RPL14 antibody 82688-1-RRDownload protocol
WB protocol for RPL14 antibody 82688-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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