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RPL14 Polyclonal antibody

RPL14 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 14991-1-AP

Print datasheet

Synonyms

60S ribosomal protein L14, CAG ISL 7, CTG B33, hRL14, L14, ribosomal protein L14, RL14, RPL14

验证数据展示

  • WB analysis using 14991-1-AP

    WB analysis using 14991-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14991-1-AP

    IHC staining of human colon cancer using 14991-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with RPL14 antibody included
  • IHC staining of human colon cancer using 14991-1-AP

    IHC staining of human colon cancer using 14991-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14991-1-AP (RPL14 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with RPL14 antibody included
  • IF Staining of HepG2 using 14991-1-AP

    IF Staining of HepG2 using 14991-1-AP

    Immunofluorescent analysis of HepG2 cells, using RPL14 antibody 14991-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IP experiment of mouse liver using 14991-1-AP

    IP experiment of mouse liver using 14991-1-AP

    IP result of anti-RPL14 (IP:14991-1-AP, 4ug; Detection:14991-1-AP 1:1000) with mouse liver tissue lysate 6800ug.

  • WB analysis using 14991-1-AP

    WB analysis using 14991-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14991-1-AP (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, MCF-7 cells, mouse liver tissue, rat liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy RPL14 Ready-To-Use IHC Kit. RPL14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14991-1-AP targets RPL14 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPL14 fusion protein Ag6976 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribosomal protein L14
Synonyms 60S ribosomal protein L14, CAG ISL 7, CTG B33, hRL14, L14, ribosomal protein L14, RL14, RPL14
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 25-30 kDa
GenBank Accession NumberBC000606
Gene Symbol RPL14
Gene ID (NCBI) 9045
RRIDAB_2285319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP50914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.

实验方案

Product Specific Protocols
WB protocol for RPL14 antibody 14991-1-APDownload protocol
IHC protocol for RPL14 antibody 14991-1-APDownload protocol
IF protocol for RPL14 antibody 14991-1-APDownload protocol
IP protocol for RPL14 antibody 14991-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Res

Pre-ribosomal RNA reorganizes DNA damage repair factors in nucleus during meiotic prophase and DNA damage response.

Authors - Xiaochen Gai
View Article
humanWB,IF

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
View Article
humanWB

Am J Cancer Res

UBE2S targets RPL26 for ubiquitination and degradation to promote non-small cell lung cancer progression via regulating c-Myc

Authors - Dalian Gong
View Article
humanCoIP,WB

Cell Discov

The BRCA1/BARD1 complex recognizes pre-ribosomal RNA to facilitate homologous recombination

Authors - Duo Wu
View Article