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验证数据展示

  • WB analysis using 16222-1-AP

    WB analysis using 16222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16222-1-AP

    WB analysis using 16222-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16222-1-AP (RPL10L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

RPL10L Polyclonal antibody

RPL10L Polyclonal Antibody for WB, ELISA
Cat No. 16222-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

60S ribosomal protein L10 like, ribosomal protein L10 like, RPL10_5_1358, RPL10L

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, mouse brain tissue, HeLa cells, SKOV-3 cells, mouse testis tissue, rat testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16222-1-AP targets RPL10L in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9275

Product name: Recombinant human RPL10L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-214 aa of BC014310

Sequence: MGRRPARCYRYCKNKPYPKSRFCRGVPDAKIRIFDLGRKKAKVDEFPLGGHMVSDEYEQLSSEALEAARICANKYMVKSCGRDGFHMRVRLHPFHVIRINKMLSCAGADRLQTGMRGAFGKPQGTVARVHIGQVIMSIRTKLQNEEHVIEALRRAKFKFPGRQKIHISKKWGFTKFNADEFEDMVAKKCLIPDGCGVKYVPSHGPLDKWRVLHS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L10-like
别名 60S ribosomal protein L10 like, ribosomal protein L10 like, RPL10_5_1358, RPL10L
计算分子量 214 aa, 25 kDa
观测分子量 25 kDa
GenBank蛋白编号BC014310
基因名称 RPL10L
Gene ID (NCBI) 140801
RRIDAB_3085482
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96L21
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RPL10L (Ribosomal Protein L10 Like), is a testis-specific component of the ribosome, is responsible for regulating the biosynthesis and folding of a subset of male germ-cell-specific proteins that are essential for the formation of sperm(Uniprot). RPL10L plays an essential role during meiosis of spermatogenesis by compensating for its X-linked parental paralog, RPL10, during and after meiotic sex chromosome inactivation(MSCI) (PMID: 28502657).

实验方案

Product Specific Protocols
WB protocol for RPL10L antibody 16222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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