RPH3A Polyclonal antibody

RPH3A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11396-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

Rabphilin 3A, Rabphilin, KIAA0985, Exophilin-1, Exophilin1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells, human brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11396-1-AP targets RPH3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 RPH3A fusion protein Ag1956 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 rabphilin 3A homolog (mouse)
别名 Rabphilin 3A, Rabphilin, KIAA0985, Exophilin-1, Exophilin1
计算分子量 690 aa, 76 kDa
观测分子量 76 kDa
GenBank蛋白编号BC017259
基因名称 RPH3A
Gene ID (NCBI) 22895
RRIDAB_2181145
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y2J0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RAB3A is a small G protein that is thought to act in exocytosis of neurotransmitters and hormones in synaptic neurotransmission and cell-cell communication. RPH3A (RPH3A), a RAB3A effector, is a neuronal C2 domain tandem containing protein that is involved in the neuronal transmitter process. Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by progressive motor disturbances, cognitive defects and dementia. Level of rabphilin 3A is substantially decreased in synapses of most brain regions in R6/1 transgenic mouse model of HD suggesting a role of RPH3A in impaired synaptic transmission.

实验方案

Product Specific Protocols
WB protocol for RPH3A antibody 11396-1-APDownload protocol
IHC protocol for RPH3A antibody 11396-1-APDownload protocol
IF protocol for RPH3A antibody 11396-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells

Authors - Tatsuaki Kurosaki
mouseWB

Front Aging Neurosci

Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses

Authors - Elena Ferrari
mouseWB

Sci Rep

Multiple Transcriptomic Analyses Explore Potential Synaptic Biomarker Rabphilin-3A for Alzheimer's Disease

Authors - Doan Phuong Quy Nguyen
ratWB

Br J Pharmacol

Memantine-induced functional rewiring of the glutamate synapse in the striatum of dopamine transporter knockout rats

Authors - Lucia Caffino
mouseWB,IF

Pharmacol Res

Rabphilin-3A as a novel target to reverse α-synuclein-induced synaptic loss in Parkinson's disease

Authors - Elena Ferrari
ratWB

Elife

Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis

Authors - Tianzhi Li
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