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RPE Polyclonal antibody

RPE Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12168-2-AP

Print datasheet

Synonyms

ribulose 5 phosphate 3 epimerase, Ribulose phosphate 3 epimerase, RPE, RPE2 1

验证数据展示

  • WB analysis of HEK-293T using 12168-2-AP

    WB analysis of HEK-293T using 12168-2-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 12168-2-AP (RPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 12168-2-AP

    IF Staining of RAW 264.7 using 12168-2-AP

    Immunofluorescent analysis of RAW 264.7 cells using 12168-2-AP (RPE antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, mouse thymus tissue, HeLa cells, K-562 cells
Positive IF detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12168-2-AP targets RPE in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RPE fusion protein Ag2810 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ribulose-5-phosphate-3-epimerase
Synonyms ribulose 5 phosphate 3 epimerase, Ribulose phosphate 3 epimerase, RPE, RPE2 1
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC016764
Gene Symbol RPE
Gene ID (NCBI) 6120
RRIDAB_2253939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AT9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RPE antibody 12168-2-APDownload protocol
IF protocol for RPE antibody 12168-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport.

Authors - Harry E Taylor
View Article
humanWB

Nat Metab

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions

Authors - Owen S Skinner
  • KO Validated
View Article