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  • Featured Product
  • KD/KO Validated

RPAP1 Polyclonal antibody

RPAP1 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 15138-1-AP

Print datasheet

Synonyms

KIAA1403, RPAP1

验证数据展示

  • WB analysis using 15138-1-AP

    WB analysis using 15138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15138-1-AP (RPAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15138-1-AP

    IP experiment of HeLa using 15138-1-AP

    IP result of anti-RPAP1 (IP:15138-1-AP, 3ug; Detection:15138-1-AP 1:800) with HeLa cells lysate 3000ug.

  • WB analysis of HEK-293 using 15138-1-AP

    WB analysis of HEK-293 using 15138-1-AP

    WB result of RPAP1 antibody (15138-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPAP1 transfected HEK-293 cells.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15138-1-AP targets RPAP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen RPAP1 fusion protein Ag7286 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RNA polymerase II associated protein 1
Synonyms KIAA1403, RPAP1
Calculated Molecular Weight 153 kDa
Observed Molecular Weight 153 kDa
GenBank Accession NumberBC000246
Gene Symbol RPAP1
Gene ID (NCBI) 26015
RRIDAB_2301137
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWH6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.

实验方案

Product Specific Protocols
WB protocol for RPAP1 antibody 15138-1-APDownload protocol
IP protocol for RPAP1 antibody 15138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Rep

The RNA Polymerase II Factor RPAP1 Is Critical for Mediator-Driven Transcription and Cell Identity.

Authors - Cian J Lynch
  • KD Validated
View Article
humanWB

Cell Rep

Mechanistic insights into COVID-19 by global analysis of the SARS-CoV-2 3CLpro substrate degradome.

Authors - Isabel Pablos
View Article