RP2 Polyclonal antibody
RP2 Polyclonal Antibody for FC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, Jurkat cells, Y79 cells, mouse liver tissue |
| Positive IP detected in | Y79 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
14151-1-AP targets RP2 in WB, IP, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Immunogen | RP2 fusion protein Ag5332 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | retinitis pigmentosa 2 (X-linked recessive) |
| Synonyms | DELXp11.3, KIAA0215, Protein XRP2, RP2, TBCCD2 |
| Calculated Molecular Weight | 350 aa, 40 kDa |
| Observed Molecular Weight | 37-40 kDa |
| GenBank Accession Number | BC043348 |
| Gene Symbol | RP2 |
| Gene ID (NCBI) | 6102 |
| RRID | AB_11182501 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75695 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RP2 antibody 14151-1-AP | Download protocol |
| IHC protocol for RP2 antibody 14151-1-AP | Download protocol |
| IP protocol for RP2 antibody 14151-1-AP | Download protocol |
| FC protocol for RP2 antibody 14151-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Life Sci A look into retinal organoids: methods, analytical techniques, and applications. | ||
Sci Rep The BEACH Protein LRBA Promotes the Localization of the Heterotrimeric G-protein Golf to Olfactory Cilia. |
