验证数据展示
经过测试的应用
Positive WB detected in | mouse testis tissue, human testis tissue, rat testis tissue |
Positive IHC detected in | mouse testis tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 1 publications below |
产品信息
20936-1-AP targets DPCD in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human |
免疫原 | DPCD fusion protein Ag15029 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | deleted in a mouse model of primary ciliary dyskinesia |
别名 | DPCD, Protein DPCD, RP11 529I10.4, RP11-529I10.4 |
计算分子量 | 203 aa, 23 kDa |
观测分子量 | 23-26 kDa |
GenBank蛋白编号 | BC031695 |
基因名称 | DPCD |
Gene ID (NCBI) | 25911 |
RRID | AB_10734461 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9BVM2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DPCD (for deleted in a mouse model of primary ciliary dyskinesia), is predicted to code for a protein of 23 kDa which remains a novel candidate gene for PCD (Primary ciliary dyskinesia).
实验方案
Product Specific Protocols | |
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WB protocol for DPCD antibody 20936-1-AP | Download protocol |
IHC protocol for DPCD antibody 20936-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Rep TDP-43 aggregation mirrors TDP-43 knockdown, affecting the expression levels of a common set of proteins. |