验证数据展示
经过测试的应用
| Positive WB detected in | A-204 cells, MG-63 cells, SH-SY5Y cells, SK-N-SH cells, SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34185-1-AP targets RP11-45B20.2 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag41415 Product name: Recombinant human RP11-45B20.2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 197-333 aa of BC137004 Sequence: LVLPKSAFTVGLTVLSKFPSSDVPIKFDKILYNEFNHYDTAVGKFTCHIAGVYYFTYHITVFSRNVQVSLVKNGVKILHTRDAYVSSEDQASGSIVLQLKLGDEMWLQVTGGERFNGLFADEDDDTTFTGFLLFSSQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | collagen triple helix repeat-containing |
| 别名 | C1q/TNF-related protein 9B, C1QTNF9B, Complement C1q and tumor necrosis factor-related protein 9B, Complement C1q and tumor necrosis factor-related protein 9-like, CTRP9B |
| 观测分子量 | 34-37 kDa |
| GenBank蛋白编号 | BC137004 |
| 基因名称 | RP11-45B20.2 |
| Gene ID (NCBI) | 387911 |
| RRID | AB_3743168 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | B2RNN3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RP11-45B20.2(C1QTNF9B/CTRP9B) is a secreted protein with triple helix structure of collagen and C1q domain, belonging to CTRP family. It may participate in metabolic regulation by activating AMPK/AKT/MAPK signaling pathway, and it may be used as a potential prognostic marker in many cancers. The expression of this protein in retina also suggests that it may be related to eye diseases (such as high myopia). Its observed molecular weight is 34-37 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RP11-45B20.2 antibody 34185-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
