验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13205-1-AP targets RORC in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, ChIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig, chicken |
| 免疫原 |
CatNo: Ag3961 Product name: Recombinant human RORC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 165-518 aa of BC031554 Sequence: DLPEASACPPGLLKASGSGPSYSNNLAKAGLNGASCHLEYSPERGKAEGRESFYSTGSQLTPDRCGLRFEEHRHPGLGELGQGPDSYGSPSFRSTPEAPYASLTEIEHLVQSVCKSYRETCQLRLEDLLRQRSNIFSREEVTGYQRKSMWEMWERCAHHLTEAIQYVVEFAKRLSGFMELCQNDQIVLLKAGAMEVVLVRMCRAYNADNRTVFFEGKYGGMELFRALGCSELISSIFDFSHSLSALHFSEDEIALYTALVLINAHRPGLQEKRKVEQLQYNLELAFHHHLCKTHRQSILAKLPPKGKLRSLCSQHVERLQIFQHLHPIVVQAAFPPLYKELFSTETESPVGLSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RAR-related orphan receptor C |
| 别名 | NR1F3, Nuclear receptor ROR-gamma, RAR-related orphan receptor C, Retinoid-related orphan receptor-gamma, ROR gamma T |
| 计算分子量 | 518 aa, 58 kDa |
| 观测分子量 | 58 kDa |
| GenBank蛋白编号 | BC031554 |
| 基因名称 | RORC |
| Gene ID (NCBI) | 6097 |
| RRID | AB_2180306 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51449 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Nuclear receptors retinoic acid receptor-related orphan receptor gamma (RORγ or RORc, also known as NR1F3) was the third and most recent ROR group member to be discovered, following the prior disclosures of RORα (RORa or NR1F1) and RORβ (RORb or NR1F2) (PMID:24502334). Studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2.RORC has 2 isoforms with the MW of 56 kDa and 58 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RORC antibody 13205-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Immunity A. faecalis induces intestinal T helper 17 cells by promoting E3 ubiquitin ligase Trim21-mediated E3 ubiquitin ligase Fbxw7 degradation | ||
Cancer Res Retinoic acid-related orphan receptor C regulates proliferation, glycolysis, and chemoresistance via the PD-L1/ITGB6/STAT3 signaling axis in bladder cancer. | ||
Phytomedicine Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa | ||
Phytomedicine Qing-Chang-Hua-Shi granule ameliorates DSS-induced colitis by activating NLRP6 signaling and regulating Th17/Treg balance | ||
Phytomedicine Salidroside alleviates bone cancer pain by inhibiting Th17/Treg imbalance through the AMPK/SIRT1 pathway | ||
Oncogene Targeting the cholesterol-RORα/γ axis inhibits colorectal cancer progression through degrading c-myc |
