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验证数据展示

  • WB analysis using 12565-1-AP

    WB analysis using 12565-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12565-1-AP (ROPN1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 12565-1-AP

    IHC staining of mouse testis using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 12565-1-AP

    IHC staining of mouse testis using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12565-1-AP

    IHC staining of human colon cancer using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ROPN1L Polyclonal antibody

ROPN1L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12565-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ropporin 1 like, ROPN1-like protein, ROPN1 like protein, ASP, AKAP-associated sperm protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12565-1-AP targets ROPN1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ROPN1L fusion protein Ag3178 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ropporin 1-like
别名 ropporin 1 like, ROPN1-like protein, ROPN1 like protein, ASP, AKAP-associated sperm protein
计算分子量 230 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC014607
基因名称 ROPN1L
Gene ID (NCBI) 83853
RRIDAB_10638785
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96C74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROPN1L (Rhophilin Associated Tail Protein 1 Like) is a protein-coding gene that is a member of the ropporin family. ROPN1L plays a role in sperm function, as mutations in ROPN1L can cause defects in the fibrous sheath integrity, sperm motility, and PKA-dependent signaling processes, leading to male infertility.

实验方案

Product Specific Protocols
WB protocol for ROPN1L antibody 12565-1-APDownload protocol
IHC protocol for ROPN1L antibody 12565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols