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验证数据展示

  • WB analysis using 12565-1-AP

    WB analysis using 12565-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12565-1-AP (ROPN1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 12565-1-AP

    IHC staining of mouse testis using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 12565-1-AP

    IHC staining of mouse testis using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12565-1-AP

    IHC staining of human colon cancer using 12565-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12565-1-AP (ROPN1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ROPN1L Polyclonal antibody

ROPN1L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12565-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

ropporin 1 like, ROPN1-like protein, ROPN1 like protein, ASP, AKAP-associated sperm protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12565-1-AP targets ROPN1L in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3178

Product name: Recombinant human ROPN1L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-230 aa of BC014607

Sequence: MPLPDTMFCAQQIHIPPELPDILKQFTKAAIRTQPADVLRWSAGYFSALSRGDPLPVKDRMEMPTATQKTDTGLTQGLLKVLHKQCHHKRYVELTDLEQKWKNLCLPKEKFKALLQLDPCENKIKWINFLALGCSMLGGSLNTALKHLCEILTDDREGGPARIPFKTFSYVYRYLARLDSDVSPLETESYLASLKENIDARKNGMIGLSDFFFPKRKLLESIENSEDVGH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ropporin 1-like
别名 ropporin 1 like, ROPN1-like protein, ROPN1 like protein, ASP, AKAP-associated sperm protein
计算分子量 230 aa, 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC014607
基因名称 ROPN1L
Gene ID (NCBI) 83853
RRIDAB_10638785
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96C74
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ROPN1L (Rhophilin Associated Tail Protein 1 Like) is a protein-coding gene that is a member of the ropporin family. ROPN1L plays a role in sperm function, as mutations in ROPN1L can cause defects in the fibrous sheath integrity, sperm motility, and PKA-dependent signaling processes, leading to male infertility.

实验方案

Product Specific Protocols
IHC protocol for ROPN1L antibody 12565-1-APDownload protocol
WB protocol for ROPN1L antibody 12565-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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