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验证数据展示

  • WB analysis using 34136-1-AP (same clone as 34136-1-PBS)

    WB analysis using 34136-1-AP (same clone as 34136-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 34136-1-AP (ROPN1B antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 34136-1-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 34136-1-AP (same clone as 34136-1-PBS)

    IHC staining of rat testis using 34136-1-AP (same clone as 34136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 34136-1-AP (ROPN1B antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 34136-1-PBS in a different storage buffer formulation.

ROPN1B Polyclonal antibody, PBS Only

ROPN1B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 34136-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Rhophilin-associated protein 1B, Ropporin-1B

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

34136-1-PBS targets ROPN1B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10787

Product name: Recombinant human ROPN1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-120 aa of BC015413

Sequence: MWKVVNLPTDLFNSVMNVGRFTEEIEWLKFLALACSALGVTITKTLKIVCEVLSCDHNGGLPRIPFSTFQFLYTYIAEVDGEICASHVSRMLNYIEQEVIGPDGLITVNDFTQNPRVWLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ropporin, rhophilin associated protein 1B
别名 Rhophilin-associated protein 1B, Ropporin-1B
计算分子量 120 aa, 24 kDa
观测分子量22 kDa
GenBank蛋白编号BC015413
基因名称 ROPN1B
Gene ID (NCBI) 152015
RRIDAB_3743160
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9BZX4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ropporin-1B (ROPN1B), cancer-restricted antigen, is highly expressed and immunogenic, inducing humoral immunity in patients with advanced metastatic melanoma (PMID: 33918976). It is important for male fertility. With ROPN1L, involved in fibrous sheath integrity and sperm motility, it plays a role in PKA-dependent signaling processes required for spermatozoa capacitation (PMID: 23303679).

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