验证数据展示
经过测试的应用
| Positive WB detected in | rat brain tissue, HeLa cells, mouse brain tissue |
| Positive IHC detected in | human liver cancer tissue, human colon tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20248-1-AP targets ROCK2-Specific(C-term) in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Rho-associated, coiled-coil containing protein kinase 2 |
| 别名 | ROCK2, EC:2.7.11.1, KIAA0619, p164 ROCK-2, Rho kinase 2 |
| 计算分子量 | 161 kDa |
| 观测分子量 | 164 kDa |
| GenBank蛋白编号 | NM_004850 |
| 基因名称 | ROCK2 |
| Gene ID (NCBI) | 9475 |
| RRID | AB_10665551 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75116 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ROCK2-Specific(C-term) antibody 20248-1-AP | Download protocol |
| IHC protocol for ROCK2-Specific(C-term) antibody 20248-1-AP | Download protocol |
| WB protocol for ROCK2-Specific(C-term) antibody 20248-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. | ||
Acta Biomater ROCK isoforms differentially modulate cancer cell motility by mechanosensing the substrate stiffness.
| ||
J Orthop Translat Augmentation of functional recovery via ROCK/PI3K/AKT pathway by Fasudil Hydrochloride in a rat sciatic nerve transection model | ||
Proc Natl Acad Sci U S A ROCK2 interacts with p22phox to phosphorylate p47phox and to control NADPH oxidase activation in human monocytes | ||
Int J Mol Med Inhibition of Rho kinase protects against colitis in mice by attenuating intestinal epithelial barrier dysfunction via MLC and the NF-κB pathway. |
