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验证数据展示

  • WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

    WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66633-1-Ig (ROCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

    WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

    WB result of ROCK2 antibody (66633-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2 transfected HeLa cells. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ROCK2 antibody included
  • Featured Product
  • KD/KO验证

ROCK2 Monoclonal antibody, PBS Only

ROCK2 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66633-1-PBS

产品说明书

CloneNo. 1C7B8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

1C7B8, EC:2.7.11.1, KIAA0619, p164 ROCK-2, Rho kinase 2

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ROCK2 Ready-To-Use IHC Kit. ROCK2 primary antibody included.

产品信息

66633-1-PBS targets ROCK2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag16330

Product name: Recombinant human ROCK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-180 aa of BC111801

Sequence: MEIDMTYQLKVIQQSLEQEEAEHKATKARLADKNKIYESIEEAKSEAMKEMEKKLLEERTLKQKVENLLLEAEKRCSLLDCDLKQSQQKINELLKQKDVLNEDVRNLTLKIEQETQKRCLTQNDLKMQTQQVNTLKMSEKQLKQENNHLMEMKMNLEKQNAELRKERQDADGQMKELQDQ

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Rho-associated, coiled-coil containing protein kinase 2
别名 1C7B8, EC:2.7.11.1, KIAA0619, p164 ROCK-2, Rho kinase 2
计算分子量 77 kDa, 161 kDa
观测分子量 161 kDa
GenBank蛋白编号BC111801
基因名称 ROCK2
Gene ID (NCBI) 9475
RRIDAB_2881992
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO75116
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.

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