验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
产品信息
20221-1-AP targets ROBO4-Specific in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | roundabout homolog 4, magic roundabout (Drosophila) |
| 别名 | ROBO4, ECSM4, Magic roundabout, MRB, Roundabout homolog 4 |
| 计算分子量 | 107 kDa |
| 观测分子量 | 90-170 kDa |
| GenBank蛋白编号 | NM_019055 |
| 基因名称 | ROBO4 |
| Gene ID (NCBI) | 54538 |
| RRID | AB_10665944 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8WZ75 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROBO4 belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for Slit proteins, at least for SLIT2, and seems to be involved in angiogenesis and vascular patterning. ROBO4 may mediate the inhibition of primary endothelial cell migration by Slit proteins. This antibody is specific to ROBO4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROBO4-Specific antibody 20221-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Mol Biol Lett Robo4 inhibits gamma radiation-induced permeability of a murine microvascular endothelial cell by regulating the junctions | ||
J Transl Med The role of TET2-mediated ROBO4 hypomethylation in the development of diabetic retinopathy | ||
Mol Ther Nucleic Acids Role of ANKHD1/LINC00346/ZNF655 Feedback Loop in Regulating the Glioma Angiogenesis via Staufen1-Mediated mRNA Decay. | ||
Science A subcellular map of translational machinery composition and regulation at the single-molecule level |
