验证数据展示
经过测试的应用
| Positive WB detected in | Neuro-2a cells, SH-SY5Y cells, Y79 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11982-1-AP targets ROBO3 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | ROBO3 fusion protein Ag2608 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | roundabout, axon guidance receptor, homolog 3 (Drosophila) |
| 别名 | Roundabout-like protein 3, RIG1, RBIG1, HGPS, HGPPS |
| 计算分子量 | 148 kDa |
| 观测分子量 | 200-250 kDa, 148 kDa |
| GenBank蛋白编号 | BC008623 |
| 基因名称 | ROBO3 |
| Gene ID (NCBI) | 64221 |
| RRID | AB_10643388 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96MS0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ROBO3 belongs to the immunoglobulin superfamily and ROBO family. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal cord development, it plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. It is required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ROBO3 antibody 11982-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |