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验证数据展示

  • WB analysis using 32844-1-AP

    WB analysis using 32844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32844-1-AP (RNPS1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RNPS1 Polyclonal antibody

RNPS1 Polyclonal Antibody for WB, ELISA
Cat No. 32844-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

E5.1, LDC2, RNA-binding protein with serine-rich domain 1, RNPS 1, SR-related protein LDC2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Raji cells, Ramos cells, fetal human brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32844-1-AP targets RNPS1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 RNPS1 fusion protein Ag37888 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding protein S1, serine-rich domain
别名 E5.1, LDC2, RNA-binding protein with serine-rich domain 1, RNPS 1, SR-related protein LDC2
计算分子量 34 kDa
观测分子量45 kDa
GenBank蛋白编号BC001838
基因名称 RNPS1
Gene ID (NCBI) 10921
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15287
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The splicing process of mRNA must be highly precise to carry the correct genetic information to the cytoplasm as templates for translation. And this process involves more than 50 proteins in the splicing complex. RNPS1 is a versatile factor that regulates alternative splicing of a number of pre-mRNAs. It participates in both constitutive splicing and in distinctive modulation of alternative splicing in a substrate dependent manner, and involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Its ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro. The molecular weight observed is consistent with what has been described in the literature (PMID: 39687031/PMID: 10449421).

实验方案

Product Specific Protocols
WB protocol for RNPS1 antibody 32844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols