验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse brain tissue, Raji cells, mouse kidney tissue, human brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10555-1-AP targets RNPS1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IF, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | RNPS1 fusion protein Ag0844 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RNA binding protein S1, serine-rich domain |
| 别名 | E5.1, LDC2, RNPS1, SR related protein LDC2 |
| 计算分子量 | 34 kDa |
| 观测分子量 | 55-60 kDa |
| GenBank蛋白编号 | BC001838 |
| 基因名称 | RNPS1 |
| Gene ID (NCBI) | 10921 |
| RRID | AB_2181682 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15287 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The splicing process of mRNA must be highly precise to carry the correct genetic information to the cytoplasm as templates for translation. And this process involves more than 50 proteins in the splicing complex. RNPS1 is a versatile factor that regulates alternative splicing of a number of pre-mRNAs. It participates in both constitutive splicing and in distinctive modulation of alternative splicing in a substrate dependent manner, and involved in UPF2-dependent nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Its ser-53 phosphorylation site is important for splicing and translation stimulation activity in vitro
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNPS1 antibody 10555-1-AP | Download protocol |
| IF protocol for RNPS1 antibody 10555-1-AP | Download protocol |
| IP protocol for RNPS1 antibody 10555-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cell AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay. | ||
J Cell Sci Novel stress granules-like structures are induced via a paracrine mechanism during viral infection. | ||
Biochem Biophys Res Commun RNPS1 inhibition aggravates ischemic brain injury and promotes neuronal death.
| ||
Int J Oral Sci RNPS1 stabilizes NAT10 protein to facilitate translation in cancer via tRNA ac4C modification
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