验证数据展示
经过测试的应用
| Positive WB detected in | 3T3-L1 cells, mouse spleen tissue |
| Positive IP detected in | mouse spleen tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
25820-1-AP targets RNPC3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag22957 Product name: Recombinant human RNPC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 169-517 aa of BC010697 Sequence: LANIVNALASVPKFYVQVLHLMNKMNLPTPFGPITARPPMYEDYMPLHAPLPPTSPQPPEEPPLPDEDEELSSEESEYESTDDEDRQRMNKLMELANLQPKRPKTIKQRHVRKKRKIKDMLNTPLCPSHSSLHPVLLPSDVFDQPQPVGNKRIEFHISTDMPAAFKKDLEKEQNCEEKNHDLPATEVDASNIGFGKIFPKPNLDITEEIKEDSDEMPSECISRRELEKGRISREEMETLSVFRSYEPGEPNCRIYVKNLAKHVQEKDLKYIFGRYVDFSSETQRIMFDIRLMKEGRMKGQAFIGLPNEKAAAKALKEANGYVLFGKPMVVQFARSARPKQDPKEGKRKC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | RNA-binding region (RNP1, RRM) containing 3 |
| 别名 | KIAA183, KIAA1839, RBM40, RNA binding motif protein 40, RNA binding protein 40, RNP, RNPC3, U11/U12 65K, U11/U12 snRNP 65 kDa protein |
| 计算分子量 | 517 aa, 59 kDa |
| 观测分子量 | 62-65 kDa |
| GenBank蛋白编号 | BC010697 |
| 基因名称 | RNPC3 |
| Gene ID (NCBI) | 55599 |
| RRID | AB_2880254 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96LT9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for RNPC3 antibody 25820-1-AP | Download protocol |
| WB protocol for RNPC3 antibody 25820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Minor Spliceosomal 65K/RNPC3 Interacts with ANKRD11 and Mediates HDAC3-Regulated Histone Deacetylation and Transcription | ||
RNA Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective Rnpc3-deficient mice.
| ||
PLoS Genet Alternative exon definition events control the choice between nuclear retention and cytoplasmic export of U11/U12-65K mRNA. |
