验证数据展示
经过测试的应用
| Positive WB detected in | MDA-MB-231 cells, SH-SY5Y cells, mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20105-1-AP targets RNF5 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | RNF5 fusion protein Ag13737 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 5 |
| 别名 | G16, NG2, Protein G16, RING5, RMA1 |
| 计算分子量 | 180 aa, 20 kDa |
| 观测分子量 | 15-50 kDa |
| GenBank蛋白编号 | BC004155 |
| 基因名称 | RNF5 |
| Gene ID (NCBI) | 6048 |
| RRID | AB_3669323 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q99942 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The RING (really interesting new gene) finger protein 5 (RNF5) is an ER-associated E3 ubiquitin ligase involved in ER-associated degradation (ERAD) for misfolded proteins.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF5 antibody 20105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |