验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HeLa cells, HepG2 cells, MCF-7 cells, HEK-293T cells, RAW 264.7 cells, C2C12 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
86740-1-RR targets RNF43 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | mouse |
| 免疫原 |
CatNo: Eg6490 Product name: Recombinant Human RNF43 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 24-197 aa of NM_001305544.1 Sequence: GFGRTGLVLAAAVESERSAEQKAIIRVIPLKMDPTGKLNLTLEGVFAGVAEITPAEGKLMQSHPLYLCNASDDDNLEPGFISIVKLESPRRAPRPCLSLASKARMAGERGASAVLFDITEDRAAAEQLQQPLGLTWPVVLIWGNDAEKLMEFVYKNQKAHVRIELKEPPAWPDY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ring finger protein 43 |
| 别名 | E3 ubiquitin-protein ligase RNF43, EC:2.3.2.27, ring finger protein 43, RING-type E3 ubiquitin transferase RNF43, RNF124 |
| 计算分子量 | 86 kDa |
| 观测分子量 | 86 kDa |
| GenBank蛋白编号 | NM_001305544.1 |
| 基因名称 | RNF43 |
| Gene ID (NCBI) | 54894 |
| RRID | AB_3745094 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q68DV7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF43 (ring finger protein 43) is a member of proteins of Goliath and Godzilla families, which bears the characteristic sequence of an ubiquitin ligase and of a trans-membrane region, encoding E3 ubiquitination kinase and with the activity of E3 ubiquitin protein ligase. RNF43 has attracted much attention in the study of Wnt/β-catenin signaling pathway because of its high structural homology with ZNRF3(zinc and ring finger protein 3). RNF43 inhibits Wnt/β-catenin signalling by ubiquitinating Frizzled receptor and targeting it to the lysosomal pathway for degradation.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF43 antibody 86740-1-RR | Download protocol |
| IHC protocol for RNF43 antibody 86740-1-RR | Download protocol |
| WB protocol for RNF43 antibody 86740-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
