验证数据展示
经过测试的应用
| Positive WB detected in | mouse ovary tissue, mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
88043-1-RR targets RNF41 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11076 Product name: Recombinant human RNF41 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-317 aa of BC032637 Sequence: MGYDVTRFQGDVDEDLICPICSGVLEEPVQAPHCEHAFCNACITQWFSQQQTCPVDRSVVTVAHLRPVPRIMRNMLSKLQIACDNAVFGCSAVVRLDNLMSHLSDCEHNPKRPVTCEQGCGLEMPKDELPNHNCIKHLRSVVQQQQTRIAELEKTSAEHKHQLAEQKRDIQLLKAYMRAIRSVNPNLQNLEETIEYNEILEWVNSLQPARVTRWGGMISTPDAVLQAVIKRSLVESGCPASIVNELIENAHERSWPQGLATLETRQMNRRYYENYVAKRIPGKQAVVVMACENQHMGDDMVQEPGLVMIFAHGVEEI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | ring finger protein 41 |
| 别名 | E3 ubiquitin-protein ligase NRDP1, EC:2.3.2.27, FLRF, NRDP1, RING finger protein 41 |
| 计算分子量 | 317 aa, 36 kDa |
| 观测分子量 | 35-40 kDa |
| GenBank蛋白编号 | BC032637 |
| 基因名称 | RNF41 |
| Gene ID (NCBI) | 10193 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H4P4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF41 acts as an E3 ubiquitin ligase to promote ErbB3 ubiquitination and degradation in cultured cells through its RING finger domain (PMID: 12411582). RNF41 acts also as a PRKN modifier that accelerates its degradation, resulting in a reduction of PRKN activity, influencing the balance of the intracellular redox state (PMID: 18541373).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF41 antibody 88043-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
