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RNF40 Polyclonal antibody

RNF40 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 15621-1-AP

Print datasheet

Synonyms

BRE1 B, BRE1B, KIAA0661, RBP95, ring finger protein 40, RNF40, STARING

验证数据展示

  • WB analysis of human brain using 15621-1-AP

    WB analysis of human brain using 15621-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15621-1-AP

    IF Staining of HeLa using 15621-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15621-1-AP (RNF40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman brain tissue, mouse liver tissue, mouse lung tissue, mouse spleen tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15621-1-AP targets RNF40 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen RNF40 fusion protein Ag8047 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 40
Synonyms BRE1 B, BRE1B, KIAA0661, RBP95, ring finger protein 40, RNF40, STARING
Calculated Molecular Weight 114 kDa
Observed Molecular Weight 114 kDa
GenBank Accession NumberBC006133
Gene Symbol RNF40
Gene ID (NCBI) 9810
RRIDAB_2253949
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75150
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

实验方案

Product Specific Protocols
WB protocol for RNF40 antibody 15621-1-APDownload protocol
IF protocol for RNF40 antibody 15621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols