×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

    WB analysis of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17810-1-AP (RNF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 17810-1-AP (same clone as 17810-1-PBS)

    WB analysis of HepG2 using 17810-1-AP (same clone as 17810-1-PBS)

    WB result of RNF4 antibody (17810-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF4 transfected HepG2 cells. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

    IP experiment of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

    IP result of anti-RNF4 (IP:17810-1-AP, 4ug; Detection:17810-1-AP 1:1500) with PC-3 cells lysate 1800 ug. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 17810-1-AP (same clone as 17810-1-PBS)

    IHC staining of mouse testis using 17810-1-AP (same clone as 17810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17810-1-AP (RNF4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RNF4 antibody included
  • IHC staining of mouse testis using 17810-1-AP (same clone as 17810-1-PBS)

    IHC staining of mouse testis using 17810-1-AP (same clone as 17810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17810-1-AP (RNF4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with RNF4 antibody included
  • Featured Product
  • KD/KO Validated

RNF4 Polyclonal antibody, PBS Only

RNF4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17810-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

E3 ubiquitin ligase RNF4, E3 ubiquitin-protein ligase RNF4, EC:2.3.2.27, Protein SNURF, RES4 26

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy RNF4 Ready-To-Use IHC Kit. RNF4 primary antibody included.

产品信息

17810-1-PBS targets RNF4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12115

Product name: Recombinant human RNF4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-190 aa of BC031935

Sequence: MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 4
别名 E3 ubiquitin ligase RNF4, E3 ubiquitin-protein ligase RNF4, EC:2.3.2.27, Protein SNURF, RES4 26
计算分子量 190 aa, 21 kDa
观测分子量 30 kDa
GenBank蛋白编号BC031935
基因名称 RNF4
Gene ID (NCBI) 6047
RRIDAB_2878443
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP78317
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein. RNF4 binds polysumoylated chains and the molecular weight of RNF4 migrate in the experiment to 35 kDa, 47 kDa, 69 kDa as ubiquitinated protein.

||
New chat

Able™

正在加载，请稍候...