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验证数据展示

  • WB analysis of HepG2 using 25132-1-AP (same clone as 25132-1-PBS)

    WB analysis of HepG2 using 25132-1-AP (same clone as 25132-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25132-1-AP (RNF38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 25132-1-AP (same clone as 25132-1-PBS)

    IHC staining of human endometrial cancer using 25132-1-AP (same clone as 25132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 25132-1-AP (same clone as 25132-1-PBS)

    IHC staining of human endometrial cancer using 25132-1-AP (same clone as 25132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 25132-1-AP (same clone as 25132-1-PBS)

    IF Staining of HepG2 using 25132-1-AP (same clone as 25132-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25132-1-AP (RNF38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

RNF38 Polyclonal antibody, PBS Only

RNF38 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 25132-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

25132-1-PBS targets RNF38 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16054

Product name: Recombinant human RNF38 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 116-465 aa of BC033786

Sequence: VSPRLLHPAAHPPQQNAVMVDIHDQLHQGTVPVSYTVTTVAPHGIPLCTGQHIPACSTQQVPGCSVVFSGQHLPVCSVPPPMLQACSVQHLPVPYAAFPPLISSDPFLIHPPHLSPHHPPHLPPPGQFVPFQTQQSRSPLQRIENEVELLGEHLPVGGFTYPPSAHPPTLPPSAPLQFLTHDPLHQEVSFGVPYPPFMPRRLTGRSRYRSQQPIPPPPYHPSLLPYVLSMLPVPPAVGPTFSFELDVEDGEVENYEALLNLAERLGEAKPRGLTKADIEQLPSYRFNPNNHQSEQTLCVVCMCDFESRQLLRVLPCNHEFHAKCVDKWLKANRTCPICRADASEVHRDSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 38
别名
计算分子量 515 aa, 58 kDa
观测分子量 43-58 kDa
GenBank蛋白编号BC033786
基因名称 RNF38
Gene ID (NCBI) 152006
RRIDAB_2879914
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0F5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RING finger protein 38 is a protein that in humans is encoded by the RNF38 gene.This gene encodes a protein with a coiled-coil motif and a RING-H2 motif (C3H2C2) at its carboxy-terminus. The RING motif is a zinc-binding domain found in a large set of proteins playing roles in diverse cellular processes including oncogenesis, development, signal transduction, and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RNF38 is widely expressed with highest levels in testis. RNF38 exists various isoforms and molecular weight of isoforms are 36, 48, 52 and 58 kDa

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