验证数据展示
经过测试的应用
| Positive WB detected in | mouse testis tissue, rat testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17471-1-AP targets RNF32 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11428 Product name: Recombinant human RNF32 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 4-221 aa of BC028120 Sequence: NKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQCPKLEDSEKEYVLDPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQVLLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLRKTVPPTDAKLR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 32 |
| 别名 | FKSG33, HSD15 |
| 计算分子量 | 362 aa, 42 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC028120 |
| 基因名称 | RNF32 |
| Gene ID (NCBI) | 140545 |
| RRID | AB_3741919 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H0A6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ring Finger Protein 32 (RNF32) is a member of the RING finger protein family, characterized by a RING domain that functions as an E3 ubiquitin ligase. RNF32 is specifically expressed in testis and ovary. Studies indicate that RNF32 is expressed during spermatogenesis, most likely in spermatocytes and/or in spermatids, suggesting a possible role in sperm formation (PMID: 11890671, PMID: 41167191).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF32 antibody 17471-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
