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验证数据展示

  • IF Staining of HepG2 using 83137-3-RR

    IF Staining of HepG2 using 83137-3-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 antibody (83137-3-RR, Clone: 230446H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of PC-3 using 83137-3-RR

    FC experiment of PC-3 using 83137-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.2 ug Rnf31 Recombinant Antibody (83137-3-RR, Clone:230446H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83137-3-RR

    Affinity and Kinetic Characterization of 83137-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83137-3-RR against Human RNF31 / HOIP were performed. The affinity constant is below 1 pM.

RNF31 / HOIP Recombinant monoclonal antibody

RNF31 / HOIP Uni-rAb® Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 83137-3-RR

产品说明书

CloneNo. 230446H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

HOIP, RNF31, 230446H1, E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inPC-3 cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83137-3-RR targets RNF31 / HOIP in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34505

Product name: Recombinant human RNF31 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 459-603 aa of BC017376

Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
亲和力
KD=5.82 x 10-10M
KOff=5.93 x 10-5M
KOn=1.02 x 105M
全称 ring finger protein 31
别名 HOIP, RNF31, 230446H1, E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31
计算分子量 1072 aa, 120 kDa
GenBank蛋白编号BC017376
基因名称 RNF31
Gene ID (NCBI) 55072
RRIDAB_3670838
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ96EP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RNF31 / HOIP antibody 83137-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols