验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16802-1-AP targets RNF26 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag10285 Product name: Recombinant human RNF26 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 239-433 aa of BC000058 Sequence: AVTVTVLHPDFTLRLATQALSQLHARPSYHRLREDVMRLSRLALGSEAWRRVWSRSLQLASWPNRGGAPGAPQGDPMRVFSVRTRRQDTLPEAGRRSEAEEEEARTIRVTPVRGRERLNEEEPPGGQDPWKLLKEQEERKKCVICQDQSKTVLLLPCRHLCLCQACTEILMRHPVYHRNCPLCRRGILQTLNVYL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger protein 26 |
| 别名 | E3 ubiquitin-protein ligase RNF26, EC:2.3.2.27, RING finger protein 26 |
| 计算分子量 | 433 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC000058 |
| 基因名称 | RNF26 |
| Gene ID (NCBI) | 79102 |
| RRID | AB_2878317 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9BY78 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNF26 is concentrated predominantly in the PN segment of the ER membrane, which corresponds with its ability to position all endosomal vesicles in the PN region of the cell. RNF26 resides in the perinuclear ER membrane, and this feature enables it to spatially restrict various endolysosomal species by attracting ubiquitin-binding vesicle adaptors through a ubiquitindependent ER MCS. RNF26 is an important E3 ubiquitin ligase that has been associated with poor prognosis in bladder cancer. And RNF26 is prevalent in normal human tissues but is upregulated in several human tumor cell lines (HL-60, HeLa S3 and SW480) (PMID: 33472082, 37519262).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RNF26 antibody 16802-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci The RNF26/CBX7 axis modulates the TNF pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC.
| ||
Cell Death Dis The FOXM1/RNF26/p57 axis regulates the cell cycle to promote the aggressiveness of bladder cancer.
| ||
Br J Cancer CDK4/6 inhibitors dephosphorylate RNF26 to stabilize TSC1 and increase the sensitivity of ccRCC to mTOR inhibitors
| ||
Am J Cancer Res RNF26-mediated ubiquitination of TRIM21 promotes bladder cancer progression
| ||
Oncogene PTPRZ1 dephosphorylates and stabilizes RNF26 to reduce the efficacy of TKIs and PD-1 blockade in ccRCC | ||
Nat Commun Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice |
