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RNF25 Polyclonal antibody

RNF25 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 24536-1-AP

Print datasheet

Synonyms

AO7, ring finger protein 25, RNF25

验证数据展示

  • WB analysis of HeLa using 24536-1-AP

    WB analysis of HeLa using 24536-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24536-1-AP (RNF25 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 24536-1-AP

    IHC staining of human cervical cancer using 24536-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24536-1-AP (RNF25 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24536-1-AP targets RNF25 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA
Tested Reactivity human
Immunogen RNF25 fusion protein Ag21369 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 25
Synonyms AO7, ring finger protein 25, RNF25
Calculated Molecular Weight 459 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC015612
Gene Symbol RNF25
Gene ID (NCBI) 64320
RRIDAB_2879594
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96BH1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF25 antibody 24536-1-APDownload protocol
IHC protocol for RNF25 antibody 24536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols