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验证数据展示

  • WB analysis of HeLa using 24536-1-AP

    WB analysis of HeLa using 24536-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 24536-1-AP (RNF25 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HCT 116 using 24536-1-AP

    IF Staining of HCT 116 using 24536-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using RNF25 antibody (24536-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IHC staining of human cervical cancer using 24536-1-AP

    IHC staining of human cervical cancer using 24536-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 24536-1-AP (RNF25 Antibody) at dilution of 1:50 (under 40x lens).

RNF25 Polyclonal antibody

RNF25 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24536-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

AO7, E3 ubiquitin-protein ligase RNF25, EC:2.3.2.27, RING finger protein AO7

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24536-1-AP targets RNF25 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag21369

Product name: Recombinant human RNF25 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 111-459 aa of BC015612

Sequence: TAMLYELIEKGKEILTDNNIPHGQCVICLYGFQEKEAFTKTPCYHYFHCHCLARYIQHMEQELKAQGQEQEQERQHATTKQKAVGVQCPVCREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGGIIDLEAERNRYFISLQQPPAPAEPESAVDVSKGSQPPSTLAAELSTSPAVQSTLPPPLPVATQHICEKIPGTRSNQQRLGETQKAMLDPPKPSRGPWRQPERRHPKGGECHAPKGTRDTQELPPPEGPLKEPMDLKPEPHSQGVEGPPQEKGPGSWQGPPPRRTRDCVRWERSKGRTPGSSYPRLPRGQGAYRPGTRRESLGLESKDGS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 25
别名 AO7, E3 ubiquitin-protein ligase RNF25, EC:2.3.2.27, RING finger protein AO7
计算分子量 459 aa, 51 kDa
观测分子量 51 kDa
GenBank蛋白编号BC015612
基因名称 RNF25
Gene ID (NCBI) 64320
RRIDAB_2879594
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96BH1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for RNF25 antibody 24536-1-APDownload protocol
IHC protocol for RNF25 antibody 24536-1-APDownload protocol
WB protocol for RNF25 antibody 24536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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