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RNF219 Polyclonal antibody

RNF219 Polyclonal Antibody for IF/ICC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 24988-1-AP

Print datasheet

Synonyms

C13orf7, ring finger protein 219, RNF219

验证数据展示

  • WB analysis of HEK-293T using 24988-1-AP

    WB analysis of HEK-293T using 24988-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 24988-1-AP (RNF219 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 24988-1-AP

    IP experiment of A431 using 24988-1-AP

    IP result of anti-RNF219 (IP:24988-1-AP, 4ug; Detection:24988-1-AP 1:300) with A431 cells lysate 1200ug.

  • IF Staining of HEK-293 using 24988-1-AP

    IF Staining of HEK-293 using 24988-1-AP

    Immunofluorescent analysis of HEK-293 cells using 24988-1-AP (RNF219 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells
Positive IP detected inA431 cells, HEK-293 cells
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 24988-1-AP is RNF219 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IP, WB,ELISA
Tested Reactivity human
Immunogen RNF219 fusion protein Ag21767 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 219
Synonyms C13orf7, ring finger protein 219, RNF219
Calculated Molecular Weight 726 aa, 81 kDa
Observed Molecular Weight 80-85 kDa
GenBank Accession NumberBC136764
Gene Symbol RNF219
Gene ID (NCBI) 79596
RRIDAB_2879832
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5W0B1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF219 antibody 24988-1-APDownload protocol
IF protocol for RNF219 antibody 24988-1-APDownload protocol
IP protocol for RNF219 antibody 24988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols