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验证数据展示

  • WB analysis of SW480 using 83658-2-RR (same clone as 83658-2-PBS)

    WB analysis of SW480 using 83658-2-RR (same clone as 83658-2-PBS)

    SW480 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83658-2-PBS in a different storage buffer formulation.

  • WB analysis of Caco-2 using 83658-2-RR (same clone as 83658-2-PBS)

    WB analysis of Caco-2 using 83658-2-RR (same clone as 83658-2-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 83658-2-RR (RNF216 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83658-2-PBS in a different storage buffer formulation.

  • IP experiment of SW480 using 83658-2-RR (same clone as 83658-2-PBS)

    IP experiment of SW480 using 83658-2-RR (same clone as 83658-2-PBS)

    IP result of anti-RNF216 (IP:83658-2-RR, 4ug; Detection:83658-2-RR 1:1000) with SW480 cells lysate 1280 ug. This data was developed using the same antibody clone with 83658-2-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83658-2-RR (same clone as 83658-2-PBS)

    FC experiment of A431 using 83658-2-RR (same clone as 83658-2-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug RNF216 Recombinant antibody (83658-2-RR, Clone:240489G2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83658-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00643-1

    Cytometric bead array standard curve of MP00643-1

    Cytometric bead array standard curve of MP00643-1, RNF216 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83658-2-PBS. Detection antibody: 83658-5-PBS. Standard: Ag0238. Range: 0.625-80 ng/mL.

  • Affinity and Kinetic Characterization of 83658-2-PBS

    Affinity and Kinetic Characterization of 83658-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83658-2-PBS against Human RNF216 was performed. The affinity constant is below 1 pM.

  • Affinity and Kinetic Characterization of 83658-2-RR

    Affinity and Kinetic Characterization of 83658-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83658-2-RR against Human RNF216 were performed. The affinity constant is below 1 pM.

RNF216 Recombinant monoclonal antibody, PBS Only (Capture)

RNF216 Uni-rAb® Recombinant Antibody for WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA
Cat No. 83658-2-PBS

产品说明书

CloneNo. 240489G2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA

别名

240489G2, E3 ubiquitin-protein ligase RNF216, EC:2.3.2.27, RING finger protein 216, RING-type E3 ubiquitin transferase RNF216

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83658-2-PBS targets RNF216 as part of a matched antibody pair:

MP00643-1: 83658-2-PBS capture and 83658-5-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, FC (Intra), IP, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag0238

Product name: Recombinant human RNF216 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 73-276 aa of BC000787

Sequence: KRRHCRSYDRRALLPAVQQEQEFYEQKIKEMAEHEDFLLALQMNEEQYQKDGQLIECRCCYGEFPFEELTQCADAHLFCKECLIRYAQEAVFGSGKLELSCMEGSCTCSFPTSELEKVLPQTILYKYYERKAEEEVAAAYADELVRCPSCSFPALLDSDVKRFSCPNPHCRKETCRKCQGLWKEHNGLTCEELAEKDDIKYRTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 216
别名 240489G2, E3 ubiquitin-protein ligase RNF216, EC:2.3.2.27, RING finger protein 216, RING-type E3 ubiquitin transferase RNF216
计算分子量 48 kDa
观测分子量57 kDa, 90-99 kDa, 106~130 kDa
GenBank蛋白编号BC000787
基因名称 RNF216
Gene ID (NCBI) 54476
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NWF9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively. Western analysis detected the ~130 kDa band as indicated in literature (PMID: 21270397).

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