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RNF216 Polyclonal antibody

RNF216 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 10185-1-AP

Print datasheet

Synonyms

ring finger protein 216, RNF216, TRIAD3, U7I1, UBCE7IP1, ZIN

验证数据展示

  • WB analysis of U2OS using 10185-1-AP

    WB analysis of U2OS using 10185-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of SW480 using 10185-1-AP

    WB analysis of SW480 using 10185-1-AP

    SW480 cells were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10185-1-AP

    WB analysis using 10185-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10185-1-AP (RNF216 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse heart tissue, U2OS cells, SW480 cells, mouse kidney tissue, rat heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10185-1-AP targets RNF216 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen RNF216 fusion protein Ag0238 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ring finger protein 216
Synonyms ring finger protein 216, RNF216, TRIAD3, U7I1, UBCE7IP1, ZIN
Calculated Molecular Weight 48 kDa
Observed Molecular Weight57 kDa, 90-99 kDa
GenBank Accession NumberBC000787
Gene Symbol RNF216
Gene ID (NCBI) 54476
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NWF9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF216/TRIAD3 is an RBR (RING-between-RING) E3 ligase that encodes for multiple isoforms that include TRIAD3A, TRIAD3B, TRIAD3C and TRIAD3D/E. RNF216 is a broadly expressed RBR E3 ligase, and loss-of-function mutations in RNF216 are linked to the neurode-generative conditions Gordon-Holmes syndrome (GHS) and Huntington-like disorders (PMID: 34998453, 35620441). RNF216 has three different molecular weights, 99 kDa, 106 kDa and 57 kDa, respectively.

实验方案

Product Specific Protocols
WB protocol for RNF216 antibody 10185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Mol Med

Xanthatin synergizes with cisplatin to suppress homologous recombination through JAK2/STAT4/BARD1 axis in human NSCLC cells.

Authors - Jian Zhang
View Article