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验证数据展示

  • WB analysis using 85630-6-RR

    WB analysis using 85630-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85630-6-RR (RNF20 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IF Staining of U-87 MG using 85630-6-RR

    IF Staining of U-87 MG using 85630-6-RR

    Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using RNF20 antibody (85630-6-RR, Clone: 243078G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

RNF20 Recombinant antibody

RNF20 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85630-6-RR

产品说明书

CloneNo. 243078G12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

BRE1, BRE1 A, BRE1A, BRE1-A, E3 ubiquitin-protein ligase BRE1A

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, HeLa cells, Jurkat cells, MCF-7 cells, HEK-293 cells, NIH/3T3 cells. C2C12 cells
Positive IF/ICC detected inU-87 MG cells
Planning an IHC experiment? We recommend our IHCeasy RNF20 Ready-To-Use IHC Kit. RNF20 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85630-6-RR targets RNF20 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 RNF20 fusion protein Ag16066 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ring finger protein 20
别名 BRE1, BRE1 A, BRE1A, BRE1-A, E3 ubiquitin-protein ligase BRE1A
计算分子量 975 aa, 114 kDa
观测分子量114 kDa
GenBank蛋白编号BC110585
基因名称 RNF20
Gene ID (NCBI) 56254
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ5VTR2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RNF20, also named as E3 ubiquitin-protein ligase BRE1A, is a 975 amino acid protein, which contains 1 RING-type zinc finger and belongs to the BRE1 family. RNF20 is a component of the RNF20/40 complex and localizes in the nucleus. RNF20 is a component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B. RNF20 is required for transcriptional activation of Hox genes and recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. RNF20/40 complex monoubiquitinates and stabilizes Eg5. Loss of RNF20/40 results in spindle assembly defects, cell cycle arrest and apoptosis. Spindle assembly role of the RNF20/40 complex, and implicates the RNF20/40-Eg5 axis in breast carcinogenesis, supporting the pursuit of these proteins as potential targets for breast cancer therapeutic interventions.

实验方案

Product Specific Protocols
WB protocol for RNF20 antibody 85630-6-RRDownload protocol
IF protocol for RNF20 antibody 85630-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols